Cleared Sample Imaging by means of Light-Sheet Fluorescence Microscopy

This webinar is geared towards researchers that intend to gain further insights about the application of light-sheet microscopy for imaging of cleared samples.

Light-sheet fluorescence microscopy serves to image a great variety of large biological samples. A sheet of laser light illuminates only a thin slice of a fluorescently labelled sample. Decoupling the illumination from the detection, typically via an orthogonal arrangement, enables fast, true volume, in-depth imaging with very low photo-damage and bleaching effects.

Clearing techniques have become a valuable tool for applications in 3D microstructure analysis of tissues (e.g. Neuroscience and Developmental biology). They modify the optical properties of usually opaque samples to render them transparent while keeping their structure and fluorescent labels intact. Clearing techniques can be combined with Light-Sheet Microscopy to make the best of them enabling fast, long-term, confocal-like optical sectioning and high-quality 3D imaging of cleared samples.

This webinar will discuss light-sheet microscopy for cleared sample imaging focusing on the advantages for neuroscience and developmental biology applications.

October 30, 2018
8AM PDT • 11AM EDT • 4PM CET

Key Topics

  • Key concepts of Light-Sheet Fluorescence Microscopy
    • Advantages of Light-Sheet microscopy over conventional fluorescence microscopy techniques
  • Light-Sheet Microscopy for cleared-sample imaging
  • Applications in Neuroscience and Developmental biology

Presenters

Dane Maxfield
Sales Product Specialist at LUXENDO GmbH, Bruker Nano Surfaces

Dr. Dane Maxfield received his Master’s degree in Applied Mathematics from the University of Utah. He then finished his Ph.D. in Biology studying under Dr. Andres Villu Maricq at the University of Utah. Dr. Maxfield is currently the LUXENDO Sales and Applications Specialist at Bruker Nano Surfaces in the U.S.

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