InVi SPIM

The light-sheet microscope optimized for long-term 3D fluorescence imaging

InVi SPIM
Key features
  • Close-to-natural environmental conditions for sensitive samples
  • Compact, vibration-free and robust design
  • Resolution down to 255 nm in xy
  • Browser-based user interface

Product overview

Dedicated to live imaging, the Luxendo InVi SPIM is a microscope that has been optimized for long-term 3D fluorescence imaging of living specimens.

Easy access to the sample chamber, precisely controlled environmental conditions, maximized photo-efficiency, and short illumination times enable long-term imaging without harming live specimens. The minimization of required specimen medium further leverages its functionality.

The optical configuration combined with the fast acquisition speed of the InVi SPIM enables 3D reconstruction, tracking of cellular and subcellular positions, and morphological analysis in real time.

Typical applications of the microscope include in toto imaging of small animal models such as whole mouse embryos, Zebrafish and Drosophila embryos, imaging of dynamic processes in mammalian cell culture applications, and even live imaging of intact and living plant models.

Adding a choice of various beam patterns – classical static Gaussian light-sheets, scanned Gaussian beams, Bessel beams and optical lattices, or even customized shapes – provides unique flexibility to optimize your bioimaging experiments.

Device design

The InVi SPIM’s compact, vibration-free and robust design provides maximal stability during your long-term experiments.

Tailored to fit your lab bench, this class 1 laser system does not require any air table or vibration compensation mechanism as all moving components are light-weighted and balanced with the motor technology. Maximal stability of focus and thermal conditions are also guaranteed. The proprietary piezo crawler stages ensure longevity and precision for a permanently accurate specimen positioning. Neither the images nor the natural growth behaviour of your sample are affected by the gentle image recording.

Illumination and Detection

The illumination optics comprise light-sheet generation by scanning a beam after passing a beam shaper unit for length and diameter adjustment.

The lenses project a light-sheet on the sample. The expandable, alignment-free light source allows a range of up to six lasers (405, 445, 488, 515, 532, 561, 594, 642, and 685 nm with 40 mW each before fiber).

The detection optics are optimized for fast, high-resolution and very sensitive imaging. The two spectral detection channels equipped with 10 position high-speed filter wheels and a motorized dichroic mirror changer enable simultaneous dual channel imaging. In addition, more than two channels can be acquired sequentially. The robust sCMOS Orca Flash 4.0 V3 cameras from Hamamatsu are well-suited for experiments that require high detection efficiency, quantification and speed.

Compared to other techniques (e.g. confocal laser scanning and spinning disc microscopy) more time points can be acquired for a deeper insight into dynamic biological processes and fast movement tracking, while reducing photodamaging effects, the downside of increased illumination in those techniques.

Electronics, microscope control and computer

LUXENDO's browser-based user interface offers a simple setup and execution of multidimensional experiments, while real-time control is handled by an embedded controller to ensure microsecond precision timing independent of the PC’s performance fluctuations.

Precise timing control of all connected devices is a prerequisite for reliable experimental outcomes. Full control of data streaming to storage as well as GPU-supported image processing further complements the overall performance.

Specifications

  • Electronics, microscope software & computer
    • Embedded microscope software with an open TCP/IP-based communication interface
    • Browser-based GUI for interactive microscope control and experiment design
    • Computer with 128 GB RAM, Intel dual 8 core CPU
    • High-speed RAID controller for data streaming, 8 × 4 TB local storage in RAID 0
    • GeForce GTX 1080 graphics card
    • 10 Gbit/s on-board Ethernet port
    • 4K UHD 40 inch display

InVi SPIM

The InVi SPIM is the first commercially available inverted light-sheet microscope.

It is perfectly suited for a great variety of samples and applications ranging from cell culture to whole embryos (e.g. Drosophila and Zebrafish).

InVi SPIM

Illumination and Detection

The InVi SPIM can achieve a resolution down to 255 nm in xy, enabling subcellular resolution in living samples free of phototoxic effects.

A Nikon CFI Plan Fluor 10x W 0.3 NA water immersion objective lens projects the light-sheet on the sample. Detection includes a high numerical aperture Nikon CFI Apo 25x W 1.1 NA water immersion objective lens. An additional magnification changer results in 31.3x and 62.5x total magnification for field view and sampling adjustment according to your experimental needs.

Illumination Detection Effective Magnification Field of View Pixel size Optical Resolution
10x/0.3 NA Nikon 25x/1.1 NA 31.3x
62.5x
420 µm
210 µm
208 nm
104 nm
255 nm

Specifications

  • Illumination optics
    • Chromatic correction from 440 to 660 nm
    • Light-sheet generation by beam scanning
    • Flexible light-sheet thickness (2 µm to 6 µm)
    • Nikon CFI Plan Fluor 10x W 0.3 NA water immersion objective lens
  • Detection optics
    • Nikon CFI Apo 25x W 1.1 NA water immersion objective lens
    • 2 spectral detection channels, each equipped with a fast filter wheel (10 positions and 50 ms switching time between adjacent positions)
    • Filters adapted to the selected laser lines
    • 2 high-speed sCMOS cameras Hamamatsu Orca Flash 4.0 V3
    • Maximum frame rate >80 fps at full frame (2048 × 2048 pixels of 6.5 µm × 6.5 µm size) and up to 500 fps at subframe cropping
    • Peak quantum efficiency (QE): 82% @ 560 nm

InVi SPIM AIM

(Advanced Illumination Module)

The Advanced Illumination Module (AIM) is a modular extension of the InVi SPIM that provides the highest level of flexibility for illuminating your sample with a light-sheet.

It expands the capabilities of the InVi SPIM: while maintaining the ease-of-use and stability of the system, it adds tailorable, interactive adaptability of the beam shape to suit the highly specific requirements of your sample. You choose what gives you the best results for your 3D high resolution imaging experiment – large field of view, high speed or optimal spatial resolution.

InVi SPIM AIM

Illumination and Detection

The InVi SPIM AIM offers a variety of illumination patterns, ranging from the classical static Gaussian light-sheet or a scanned Gaussian beam to sophisticated illumination schemes like Bessel beams, Airy beams or optical lattices.

The user can select from a broad choice of beam shapes to improve the microscope’s resolution and reduce photo-damage in delicate samples. A Special Optics 28.6x 0.7 NA water immersion objective lens projects the light-sheet on the sample. A high numerical aperture Nikon CFI Apo 25x W 1.1 NA water immersion objective lens images the signal onto one or two Hamamatsu sCMOS cameras. An additional magnification changer provides 31.3x and 62.5x total magnification to allow you to optimize field of view and pixel size to your experimental needs.

Illumination Detection Effective Magnification Field of View Pixel size Optical Resolution
28.6x/0.7 NA Nikon 25x/1.1 NA 31.3x
62.5x
420 µm
210 µm
208 nm
104 nm
255 nm

Specifications

  • Illumination optics
    • Chromatic correction from 440 to 660 nm
    • Light-sheet generation with static illumination or by beam scanning
    • Flexible light-sheet geometries: static and scanned Gaussian beams, Bessel and Airy beams and optical lattices for improved resolution, field of view and speed
    • Special Optics 28.6x 0.7 NA water immersion objective lens
  • Detection optics
    • Nikon CFI Apo 25x W 1.1 NA water immersion objective lens
    • 2 spectral detection channels, each equipped with a fast filter wheel (10 positions and 50 ms switching time between adjacent positions)
    • Filters adapted to the selected laser lines
    • 2 high-speed sCMOS cameras Hamamatsu Orca Flash 4.0 V3
    • Maximum frame rate >80 fps at full frame (2048 × 2048 pixels of 6.5 µm × 6.5 µm size) and up to 500 fps at subframe cropping
    • Peak quantum efficiency (QE): 82% @ 560 nm

Need help?

LUXENDO support

If you have any question regarding your microscopes functionality or you want to schedule a maintenance visit, then get in contact with LUXENDO’s support team. We will ensure to get the right person involved to answer your request.

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