The light-sheet company

Revolutionizing bioimaging with light-sheets

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MuVi-SPIM Imaging Life In The Fast Lane

Latest sCMOS camera technology and optimized combinations of objective lenses enable you to obtain the best from your sample: data you always wanted but could never get.

  • Large field of view
  • Close-to-confocal resolution
  • Very high imaging speed
  • Highest sensitivity and minimal noise

Fruit fly development in 3D

A fruit fly embryo from when it was about two-and-a-half hours old until it walked away from the microscope as a larva.

The Microscope Seeing Life From A Different Angle – Or Four

The MuVi-SPIM provides four simultaneous orthogonal views on large living specimens without the need for sample rotation. This avoids shadowing effects and facilitates long-term imaging at dramatically increased acquisition speed. The modular software concept allows the flexible design of complex experimental layouts. 3D image data can be directly streamed to a storage and data processing server for fast data registration and fusion.

  • 3D imaging of large specimen with light-sheet illumination
  • Gentle sample handling and low phototoxicity
  • Highly sensitive and fast imaging

MuVi-SPIM The Technology

The MuVi-SPIM utilizes a sheet of laser light to illuminate only a thin slice of a fluorescently labeled sample. A wide-field fluorescence microscope, placed perpendicular to the light-sheet, serves to collect the fluorescence signal and images the observed region by means of a camera.

This features the following advantages:

  • Intrinsic 3D optical sectioning
  • Excellent signal-to-noise ratio
  • High time resolution
  • Drastically reduced overall photobleaching and phototoxicity

The Technology 360° Illumination and detection

The four objective lenses allow four combinations of illumination and detection. This provides four orthogonal views of the specimen without the need for sample rotation, enabling:

  • Multiple view imaging of large samples at unprecedented speed
  • Long-term stability
  • Time series over several days
  • Robust image registration
  • Efficient data fusion and visualization

Specifications & Configurations

Laser
  • Laser combiner with six laser positions
  • Available laser lines 445, 488, 515, 532, 561, 594, 642, and 685 nm with 50 mW each
  • Fast modulation and high extinction
Illumination optics
  • Two identical illumination arms
  • Chromatic correction from 440 to 660 nm
  • Light-sheet generation by beam scanning
  • Variable light-sheet thickness (optional)
  • Water-dipping objective lenses (see below)
Detection optics
  • Two identical detection arms
  • Water-dipping objective lenses (see below)
  • Fast filter wheels with 10 positions and 50 ms between adjacent positions
  • Filters adapted to the selected laser lines
  • Variable magnification (optional)
  • High-speed sCMOS cameras Hamamatsu Orca Flash 4.0 with 2048 x 2048 pixels of 6.5 µm x 6.5 µm size
Objective lens mounting unit
  • High precision mounting unit
  • Fast exchange of the unit with high precision
  • Mounting chamber with temperature control (see below)
  • 2 x Nikon CFI Plan Fluor 10XW 0.3 NA water immersion for illumination
  • 2 x Nikon CFI Apo LWD 25XW 1.1 NA water immersion for detection
  • Other objective lenses on request
Sample chamber and stage
  • Water-sealed inert PEEK plastic chamber, autoclavable and biocompatible
  • Sample supported from below for improved stability
  • Easy access from above for sample mounting, injections, etc.
  • Fast and precise temperature control, range 15-40°C
  • High-precision XYZ and fast rotation stage
  • XYZ piezo crawler stage with 100 nm resolution and 8.5 mm (X) and 1.2 mm (YZ) travel range for fast sample positioning and stack acquisition
  • Custom-made rotation stage with > 360 degrees/second speed
Electronics, Computer, Software
  • All electronic components included in the microscope housing
  • Embedded microscope software with open communication interface
  • Open GUI control for interactive control and microscope automation
  • Computer with 64 GB RAM, Intel Dual Six Core CPU
  • High-speed RAID controller for data streaming
  • 8 x 4 TB local storage in RAID 0
  • One 40 inch monitor

Interested in MuVi-SPIM and our follow-on products?

Just fill out the form below and our professional team will assist and help you through all your questions and needs.